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Published on: February 1, 2017
[Recombinant adenovirus-mediated expression of 1.2-copy hepatitis B virus DNA in three hepatocytes]
Wei-fang Liang1, Zhi-hua Liu, Kang-xian Luo
1Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. weifl@fimmu.com
Objective:
To study hepatitis B virus (HBV) expression in 3 hepatocytes infected with recombinant adenovirus containing 1.2-copy HBV DNA.a
Methods:
A chicken hepatoma cell line and two human hepatocytes were infected by the recombinant adenovirus containing 1.2-copy HBV DNA at 25 pfu/cell. HBV-specific mRNA was detected by RT-PCR 3 days after the infection, and HBsAg and HBeAg were detected by ELISA and HBV DNA by real-time PCR daily after the infection.
Results:
HBV mRNA expression was detected in all the 3 cells after recombinant adenovirus infection, and the quantities of HBV DNA and HBV antigens in the culture supernatant increased with the passage of time.a
Conclusion:
Infection with the recombinant adenovirus containing 1.2-copy HBV DNA can mediate HBV infection in the 3 cells in vitro.
