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Updated: Jun 23, 2026
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
[Detection of miR-122a and miR-224 expression in hepatocellular carcinoma by real-time fluorescence quantitative
Zhuo-ran Liu1, Xiao-wei Chen, Xin-hui Qiao
1Department of Clinical Laboratory, Second Affiliated Hospital, University of South China, Hengyang 421001, China. LZr9656@163.com
Objective:
To detect the expressions of miR-122 and miR-224 in hepatocellular carcinoma (HCC) by real-time fluorescence quantitative RT-PCR and investigate the significance of miRNAs in early diagnosis of HCC.
Method:
2(-Delta Delta CT) method was used for quantitative analysis of the expression pattern of miR-122 and miR-224 in 35 HCC and adjacent normal tissues. All the quantitative results were confirmed by Northern blotting.
Results:
Compared with adjacent normal tissues, the HCC tissues showed significant miR-122 down-regulation (P<0.01) and miR-224 over-expression (P<0.001).
Conclusions:
HCC has obvious alteration in the expression patterns of miR-122 and miR-224, and real-time fluorescence quantitative RT-PCR provide a new means for early, efficient, and accurate diagnosis of HCC.
