Related Experiment Video
Updated: Jan 30, 2026
Author Spotlight: Optimizing the Neurovascular Development of Human Brain Organoid in Chick Embryo
Published on: February 16, 2024
[Small interference RNA targeting nuclear factor-kappaB inhibits endometriotic angiogenesis in chick embryo
Mu-biao Liu1, Yuan-li He, Jie Zhong
1Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. liumb1972@126.com
Objective:
To investigate the effect of small interference RNA (siRNA) targeting nuclear factor-kappaB (NF-kappaB) on endometriosis.
Method:
The eutopic endometrium of women with endometriosis were transplanted into the nonvascular region of 8-day-old chicken embryo chorioallantocic membrane (CAM), and the effects of NF-kappaB p65 siRNA on the vascularization and endometriotic lesion formation were tested with proper controls.
Results:
Transplantation of the endometrium onto the CAM resulted in a strong angiogenic response in the chicken tissue. The angiogenesis was significantly reduced and endometriotic lesion formation significantly suppressed with siRNA targeting NF-kappaB in comparison with the control group.
Conclusions:
The NF-kappaB pathway is involved in the development of endometriotic lesions in vitro, and NF-kappaB gene silencing reduces endometriotic angiogenesis and promotes cell apoptosis in the endometriotic lesions, suggesting that NF-kappaB might be a good target for endometriosis treatment.
Related Concept Videos
01:23RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:31Nuclear Export of mRNA
02:36RNA Polymerase II Accessory Proteins
02:18Interference and Diffraction
03:20Nuclear Transmutation
00:46Feedback Inhibition