Related Experiment Video

Updated: Jun 23, 2026

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
09:16

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

Smurf1 zaps the talin head

David R Critchley

    Nature Cell Biology
    |May 1, 2009
    PubMed

    Insights

    The talin head fragment, released by calpain II, is crucial for cell migration. Its levels are controlled by Smurf1-mediated ubiquitylation and Cdk5-mediated phosphorylation.

    Area of Science:

    • Cell biology
    • Molecular mechanisms of cell migration
    • Protein regulation

    Background:

    • Focal adhesion turnover is a dynamic process critical for cell migration.
    • The talin protein plays a significant role in focal adhesion dynamics.
    • Calpain II is a calcium-dependent protease involved in protein cleavage.

    Discussion:

    • The liberated talin head fragment, generated by calpain II cleavage of talin, is identified as a key regulator of focal adhesion turnover.
    • This fragment's activity is modulated by specific post-translational modifications.
    • The interplay between ubiquitylation and phosphorylation dictates the fragment's stability and function.

    Key Insights:

    • Calpain II cleavage releases a functional talin head fragment essential for cell migration.
    • Smurf1-mediated ubiquitylation targets the talin head for degradation, regulating its levels.
    • Cdk5-mediated phosphorylation counteracts Smurf1 activity, stabilizing the talin head fragment.

    Outlook:

    • Further investigation into the precise mechanisms of talin head regulation could reveal new therapeutic targets for diseases involving cell migration.
    • Understanding the spatial and temporal control of focal adhesion dynamics offers insights into developmental processes.
    • Exploring the crosstalk between ubiquitylation and phosphorylation pathways in focal adhesion turnover is warranted.

    More Related Videos

    Two-Photon Calcium Imaging of Forebrain Activity in Behaving Adult Zebrafish07:21

    Two-Photon Calcium Imaging of Forebrain Activity in Behaving Adult Zebrafish

    Published on: July 28, 2023

    Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity07:31

    Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

    Published on: August 23, 2022

    Related Concept Videos

    Taping Over Different Ground Profiles01:12

    Taping Over Different Ground Profiles

    Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
    Mortar01:29

    Mortar

    Mortar, a mixture of Portland cement, hydrated lime, sand, and water, is a crucial binding material in construction. Its primary function is to join masonry units together, filling gaps and ensuring a uniform distribution of weight across the structure. This helps in preventing potential weaknesses. Mortar also serves as a protective barrier against environmental elements such as water and wind, thereby safeguarding the interior of the structure. It also compensates for surface irregularities...
    Hydraulic Jump01:29

    Hydraulic Jump

    A hydraulic jump is a sudden rise in fluid depth in open channels, occurring when high-velocity (supercritical) flow transitions to low-velocity (subcritical) flow. This phenomenon requires an upstream Froude number greater than 1, as flows with Fr1<1 remain subcritical, making a hydraulic jump impossible due to the need for negative head loss, which violates thermodynamic principles.The characteristics of a hydraulic jump depend on the upstream Froude number and are classified as...
    Flail Chest-II01:26

    Flail Chest-II

    Managing flail chest, a condition characterized by a segment of the chest wall moving independently from the rest of the thoracic cage, requires a comprehensive approach. It includes a thorough assessment of the patient's condition, a diagnostic evaluation to determine the extent of the injury, and the implementation of appropriate medical interventions tailored to the individual's needs.
    Assessment:
    1. Clinical Evaluation:
    History:
    Flail Chest-I01:24

    Flail Chest-I

    Overview of Flail Chest
    Flail chest is a severe and potentially life-threatening condition characterized by the fracture of three or more adjacent ribs in multiple places. It is most commonly caused by direct impacts and trauma, such as motor vehicle accidents or injuries from a steering wheel impact. It can also occur due to falls in elderly individuals with osteoporosis, or assaults involving sharp objects.
    Pathophysiology
    The pathophysiology of flail chest is complex, involving fractures of...
    Tongue01:01

    Tongue

    The human tongue is a fascinating and complex organ, responsible for various essential functions such as swallowing, speech, and taste. It is also subject to various conditions and diseases. In this article, we delve into the anatomy of the tongue, its roles, and some common conditions that can affect it.
    Anatomical Position in the Oral Cavity
    The tongue is located within the oral cavity, also known as the mouth. It is attached to the floor of the mouth by a fold of mucous membrane called the...