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Updated: Jun 23, 2026

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
Published on: August 20, 2009
An optical coherence microscope for 3-dimensional imaging in developmental biology
Abstract:
An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 10 mm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.
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