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Updated: Jun 23, 2026
Bridging the Bio-Electronic Interface with Biofabrication
Published on: June 6, 2012
Improvement of chloroperoxidase stability by covalent immobilization on chitosan membranes
Li-Hua Zhang1, Chao-Hong Bai, Ying-Song Wang
1School of Chemistry and Materials Science, Shaanxi Normal University, Chang'an South Road, 199, Xi'an, Shaanxi, 710062, People's Republic of China.
Abstract:
Chloroperoxidase (CPO) from Caldariomyces fumago was optimally covalently immobilized on chitosan membranes pretreated with 0.8 M glutaraldehyde at pH 3.5 to give 3.18 mg CPO g(-1) support. Using monochlorodimedone (MCD) as assay substrate, the immobilized-CPO retained 40% activity at 50 degrees C after 40 min whereas free CPO retained only 0.02%. The residual activity for immobilized-CPO was 99 and 58% compared with 68 and 43% for free CPO in the presence of 1.5 M urea and 300 microM H(2)O(2), respectively, after 20 h.
