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Updated: Jun 23, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
On-column refolding of corticotropin-releasing factor receptor 1 extracellular domain by size exclusion
Maria Ersoy1, Sandra Matic, Stefan R Schmidt
1Global Protein Science and Supply, AstraZeneca R&D Södertälje, SE-15185 Södertälje, Sweden. Stefan.Schmidt@astrazeneca.com
Abstract:
Expressing the extracellular domain of corticotropin releasing factor receptor 1 in Escherichia coli usually results in the formation of inclusion bodies. Here we describe the optimization of refolding by applying size exclusion chromatography with a denaturing guanidine hydrochloride gradient and a refolding buffer containing glycerol. Several chromatographic parameters like gradient length, flow rate, sample concentration and chromatography resin characteristics were evaluated. Recovery yields of refolded protein above 50% using a Superdex 200 column demonstrate the usefulness of this method.
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