Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial transcriptome for cell segmentation.

Nature computational science·2026
Same author

Hardware-Attentive Programmable Fourier Ptychography Enables Task-Adaptive Label-Free Virtual Staining.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Correction to "Thiolate DNAzymes on Gold Nanoparticles for Isothermal Amplification and Detection of Mesothelioma-derived Exosomal PD-L1 mRNA".

Analytical chemistry·2026
Same author

Binocular vision fusion enhanced 3D NIR-II <i>in vivo</i> imaging of bone and vessel networks.

Fundamental research·2026
Same author

Beads-on-a-Tip testing for ultrasensitive antigen detection across a large dynamic range.

Smart molecules : open access·2026
Same author

Lattice and ligand engineering for hierarchical heterogeneous nanocrystals.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Jun 23, 2026

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

Calibration beads containing luminescent lanthanide ion complexes.

Robert C Leif1, Sean Yang, Dayong Jin

  • 1Newport Instruments, 5648 Toyon Road, San Diego, California 92115-1022, USA. rleif@rleif.com

Journal of Biomedical Optics
|May 2, 2009
PubMed
Summary

New europium-complex calibration beads enhance lanthanide luminescence measurements. These beads offer reliable spectral calibration for flow cytometry and microscopy, improving measurement accuracy.

More Related Videos

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
08:31

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

Related Experiment Videos

Last Updated: Jun 23, 2026

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
08:31

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Lanthanide luminescence measurements require reliable calibration standards.
  • Current methods lack narrowband emitting, UV-excited calibration beads.

Purpose of the Study:

  • To develop and characterize europium-complex-based calibration beads.
  • To assess their suitability for flow cytometry and digital microscopy.

Main Methods:

  • Manufacturing of 0.5-, 3-, and 5-micrometer europium-complex beads.
  • Characterization using luminescence flow cytometry, confocal microscopy, PARISS spectrophotometry, and fluorescence microscopy.
  • Measurement of luminescence distribution, spatial homogeneity, emission peaks, photobleaching kinetics, and luminescence lifetimes.

Main Results:

  • Beads exhibit homogeneous europium-complex distribution.
  • Emission peaks at 592, 616 (9.9 nm width), and 685 nm.
  • Luminescence flow cytometry shows a 7.0% coefficient of variation for 5-micrometer beads.
  • Photobleaching does not impede imaging; luminescence lifetimes are 340 µs (water) and 460 µs (air).

Conclusions:

  • Developed europium-complex beads serve as effective spectral calibration standards.
  • They are suitable as test particles for time-delayed luminescence flow cytometers.
  • Potential applications include labeling macromolecules and cells.