Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advancing next-generation brain organoid platforms for investigating traumatic brain injury from repeated blast exposures.

Frontiers in bioengineering and biotechnology·2025

Same author

Optical approaches for neurocritical care: Toward non-invasive recording of cerebral physiology in acute brain injury.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2025

Same author

High-resolution transcranial optical imaging of in vivo neural activity.

Scientific reports·2024

Same author

The dynamic response of human lungs due to underwater shock wave exposure.

PloS one·2024

Same author

Sampling rate requirement for accurate calculation of heart rate and its variability based on the electrocardiogram.

Physiological measurement·2024

Same author

Erythrocyte aggregation in sudden flow arrest is linked to hyperthermia, hypoxemia, and band 3 availability.

Journal of thrombosis and haemostasis : JTH·2022

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

Uniform-intensity, visible light source for in situ imaging.

Eyal Bar-Kochba¹, Saagar Govil, Jon P Longtin

¹Stony Brook University, Department of Materials Science and Engineering, Stony Brook, New York 11794-2275, USA.

Journal of Biomedical Optics

|May 2, 2009

Summary

Researchers developed a flexible, low-cost light source for biological imaging. This thin, adaptable device uses a silica-particle-infused gel in a plastic pouch for uniform illumination in various imaging applications.

More Related Videos

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

Published on: June 2, 2023

Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs

Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO₂ Foam Transport in Fractured Unconventional Reservoirs

Published on: July 2, 2020

Related Experiment Videos

Last Updated: Jun 23, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

Published on: June 2, 2023

Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs

Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO₂ Foam Transport in Fractured Unconventional Reservoirs

Published on: July 2, 2020

Area of Science:

Biomedical Engineering
Optical Imaging
Materials Science

Background:

Standard illumination methods for biological and biomedical imaging can be costly, rigid, and generate significant heat.
There is a need for adaptable, low-cost, and high-brightness light sources suitable for in situ and in vitro specimen imaging.

Purpose of the Study:

To design, fabricate, and evaluate a novel flexible, low-cost, high-brightness light source for biological and biomedical imaging applications.
To demonstrate the device's utility in imaging biological tissues, such as hamster cheek pouch, and compare its performance to existing methods.

Main Methods:

A custom-sized, thin (1-3 mm) square plastic pouch (10-20 mm sides) was fabricated.
The pouch was filled with a silicone-based gel embedded with silica particles to act as a light-scattering medium.
Light was delivered via a multimode optical fiber connected to a high-intensity tungsten lamp.

Main Results:

The device produced a reasonably uniform, planar light source with a nearly white light spectrum.
The flexible light source exhibited a low temperature rise (<2°C) during operation.
Preliminary imaging results in hamster cheek pouch tissue showed comparable performance to standard intravital microscopy.

Conclusions:

The developed flexible light source offers a low-cost, adaptable, and effective illumination solution for biological and biomedical imaging.
The device's thin, flexible form factor and uniform light output present advantages over conventional illumination systems.
Further improvements and diverse applications in microscopy and in vivo imaging are anticipated.