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Related Experiment Video

Updated: Jun 23, 2026

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
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Visualizing laser-skin interaction in vivo by multiphoton microscopy.

Tsung-Hua Tsai1, Shiou-Hwa Jee, Jung-Yi Chan

  • 1Far Eastern Memorial Hospital, Department of Dermatology, 21, Nan-Ya South Road, Section 2, Pan-Chiao, Taipei 220, Taiwan.

Journal of Biomedical Optics
|May 2, 2009
PubMed
Summary

Multiphoton microscopy effectively monitors laser-skin interactions in vivo. This technique visualizes stratum corneum disruption and collagen damage, aiding laser-assisted drug delivery research.

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Area of Science:

  • Biomedical Optics
  • Dermatology
  • Laser-Tissue Interaction

Background:

  • Multiphoton microscopy (MPM) is a popular noninvasive imaging technique in biomedical research.
  • Understanding laser-skin interactions is crucial for applications like laser-assisted drug delivery.

Purpose of the Study:

  • To evaluate the potential of MPM for monitoring laser-induced skin reactions in vivo.
  • To assess MPM's capability in characterizing laser-induced alterations in skin structures.

Main Methods:

  • Nude mouse skin was irradiated with an erbium:YAG laser at various fluences.
  • Immediate imaging was performed using a multiphoton microscope.
  • Morphological and quantitative analysis of nonlinear optical properties (autofluorescence, second-harmonic generation) was conducted.

Main Results:

  • Low-fluence erbium:YAG laser selectively disrupted the stratum corneum, potentially enhancing transcutaneous drug delivery.
  • Higher fluences revealed tissue ablation zones and damage to stratum corneum, keratinocytes, and dermal extracellular matrix.
  • Second-harmonic generation quantified collagen damage, showing significant differences between control and laser-irradiated skin (P<0.05).

Conclusions:

  • Multiphoton microscopy is a valuable noninvasive tool for monitoring laser-skin reactions.
  • MPM provides detailed morphological and quantitative insights into laser-induced skin damage.
  • The findings support MPM's utility in optimizing laser parameters for dermatological applications.