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Related Concept Videos

Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

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Related Experiment Video

Updated: Jun 23, 2026

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Three-dimensional epithelial tissues generated from human embryonic stem cells.

Kyle J Hewitt1, Yulia Shamis, Mark W Carlson

  • 1Department of Oral and Maxillofacial Pathology, Tufts University, Boston, Massachusetts 02111, USA.

Tissue Engineering. Part A
|May 2, 2009
PubMed
Summary
This summary is machine-generated.

Human embryonic stem (hES) cells can be differentiated into distinct cell types for tissue engineering. These engineered tissues mimic natural human epithelium, offering a promising alternative to traditional cell sources.

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Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
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Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip

Published on: February 17, 2023

Related Experiment Videos

Last Updated: Jun 23, 2026

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
10:04

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
09:46

Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip

Published on: February 17, 2023

Area of Science:

  • Stem Cell Biology
  • Tissue Engineering
  • Developmental Biology

Background:

  • Pluripotent human embryonic stem (hES) cells offer advantages for tissue engineering due to their differentiation potential and proliferative capacity.
  • Traditional progenitor cells have limitations in availability and differentiation potential.

Purpose of the Study:

  • To derive distinct ectodermal and mesenchymal cell populations from hES cells.
  • To fabricate three-dimensional (3D) epithelial tissues using these derived cell populations.
  • To investigate environmental cues influencing hES cell differentiation for tissue development.

Main Methods:

  • Two-dimensional (2D) culture of hES cells to derive mesenchymal (EDK1) and epithelial (HDE1) cell populations.
  • Incorporation of derived cells into 3D tissue constructs.
  • Analysis of cell morphology, surface markers, and gene expression (cytokeratin 12, beta-catenin).
  • Assessment of basement membrane formation (type IV collagen) and epithelial stratification.

Main Results:

  • Specific culture conditions yielded EDK1 (mesenchymal) and HDE1 (epithelial) cells from hES cells.
  • HDE1 cells expressed cytokeratin 12 and beta-catenin, indicating epithelial differentiation.
  • 3D tissues fabricated with hES-derived cells formed multilayer epithelia resembling native tissues.
  • Engineered tissues exhibited fibroblast-like stromal cells, a basement membrane, and stratified epithelium.

Conclusions:

  • Distinct cell lineages can be derived from a common hES cell source for complex tissue fabrication.
  • Environmental cues play a critical role in directing hES cell differentiation towards specific tissue forms and functions.
  • hES cell-derived tissues show potential for regenerative medicine and disease modeling.