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Updated: Jun 23, 2026

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
Published on: July 19, 2021
Ultrastructural study of archaeological Vitis vinifera L. seeds using rapid-freeze fixation and substitution
Claudio Milanesi1, Rita Vignani, Andrea Ciacci
1Environmental Science G. Sarfatti Department, University of Siena, Siena, Italy. milanesi@unisi.it
Abstract:
The ultrastructure of Vitis vinifera seeds from different archaeological sites was studied. Preservation status differed between sites. Preliminary investigations of grape seeds from Poggio Bacherina (Chianciano Terme, Siena) and Miranduolo (Chiusdino, Siena) showed collapsed or charred tegument, making this material suitable for morphometric studies only. Rapid-freeze fixation and substitution of grape seeds from Shahr-I Sokhta in Iran and via De' Castellani in Florence revealed well preserved tegument suitable for chemical and cytochemical analysis. Energy dispersive X-ray microanalysis was used to determine chemical composition. Cytochemical analysis based on fluorescent staining with DAPI suggested the presence of cytoplasm residues.
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