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Updated: Jun 23, 2026
06:34
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Microbacterium binotii sp. nov., isolated from human blood
D Clermont1, S Diard, C Bouchier
1Collection de l'Institut Pasteur (CIP), Paris, France. dclermon@pasteur.fr
Summary
Two novel bacterial strains from human blood, identified as Microbacterium binotii sp. nov., were characterized using chemotaxonomic and genetic analyses. These findings establish a new species within the Microbacterium genus.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Genomics
Background:
- Two non-spore-forming, Gram-positive bacterial strains (CIP 101303(T) and CIP 102116) were isolated from human blood.
- These strains exhibited chemotaxonomic markers aligning with the genus Microbacterium.
Purpose of the Study:
- To classify and characterize two bacterial isolates from human blood.
- To determine if the isolates represent a new species within the Microbacterium genus.
Main Methods:
- Chemotaxonomic analyses including menaquinone profiling, cellular fatty acid analysis, cell-wall sugar composition, and peptidoglycan diamino acid determination.
- DNA G+C content determination.
- 16S rRNA gene sequencing and DNA-DNA relatedness studies.
Main Results:
- Chemotaxonomic data (menaquinones, fatty acids, cell-wall sugars, diamino acid) were consistent with the genus Microbacterium.
- 16S rRNA gene sequencing placed the strains within the Microbacterium genus, closely related to Microbacterium halotolerans.
- DNA-DNA relatedness indicated the isolates constitute a distinct genomic species.
Conclusions:
- The phenotypic and genotypic data support the establishment of a new species, Microbacterium binotii sp. nov.
- Strain CIP 101303(T) is proposed as the type strain for Microbacterium binotii.
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