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Updated: Jun 23, 2026

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
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Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

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Survivin expression quantified by Image Pro-Plus compared with visual assessment.

Chao-Jie Wang1, Zong-Guang Zhou, Annica Holmqvist

  • 1Institute of Digestive Surgery, Department of Colorectal Surgery, and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

Applied Immunohistochemistry & Molecular Morphology : AIMM
|May 2, 2009
PubMed
Summary

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Image analysis software like Image Pro-Plus (IPP) offers a more reliable and reproducible method for quantifying immunohistochemistry staining compared to manual visual assessment. This advanced technique provides greater sensitivity for analyzing protein expression and its relation to clinicopathologic variables in rectal cancer.

Area of Science:

  • Oncology
  • Pathology
  • Biomedical Imaging

Background:

  • Immunohistochemistry (IHC) is vital for disease diagnosis and prognosis.
  • Manual IHC interpretation and scoring are subjective and lack reproducibility.
  • Objective quantification methods are needed to improve IHC analysis.

Purpose of the Study:

  • To compare the quantitative accuracy of Image Pro-Plus (IPP) software with visual assessment for survivin immunohistochemical staining in rectal cancer.
  • To evaluate the correlation between IPP parameters and visual scoring.
  • To determine the utility of IPP in analyzing protein expression in relation to clinicopathologic variables.

Main Methods:

  • 98 rectal cancer samples were analyzed for survivin expression using IHC.
  • Image Pro-Plus (IPP) software quantified staining using density mean, area sum, and integrated optical density (IOD).

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Last Updated: Jun 23, 2026

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  • Results were compared with manual visual assessment (staining intensity and percentage).

    • Main Results:

    • Significant correlations were found between IPP parameters (density mean, IOD, area sum) and visual assessment parameters (intensity, percentage).
    • IPP analysis revealed higher survivin expression (density mean and IOD) in better-differentiated rectal cancers, unlike visual assessment.
    • Substantial agreement was observed between IPP and visual assessment methods.

    Conclusions:

    • Image Pro-Plus (IPP) provides a reliable and reproducible method for quantifying immunohistochemical staining.
    • IPP analysis offers increased sensitivity and objectivity compared to manual scoring.
    • IPP analysis yields more comprehensive insights into protein expression and its correlation with clinicopathologic variables in rectal cancer.