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Updated: Jun 23, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Enumeration of illumination and scanning modes from real-time spatial light modulators
Abstract:
Using a phase-only spatial light modulator (SLM) in a Fourier transform setup together with fast diffractive optics design algorithms provides a way to automatically generate complex and rapidly changing laser illumination patterns in the far-field. We propose a hierarchical software structure for the adaptive, on-line design of far-field illumination patterns. Using the on-line design system together with camera feedback of the illuminated scene would make it possible to detect and actively laser designate multiple objects in parallel. Possibilities for multispot, arbitrary trajectory scanning and also broad-area speckle-reduced illumination are demonstrated with experimentally measured diffraction pattern sequences from a 120 x 128 pixel phase-only SLM.
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