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Updated: Jun 23, 2026
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Detection of TT virus by single-primer sequence-independent amplification in multiple samples collected from an
S Braham1, M Iturriza-Gómara, J Gray
1Enteric Virus Unit, Virus Reference Department, Centre for Infections, Health Protection Agency, Colindale, London, NW9 5HT, UK.
Abstract:
A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.
