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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
Published on: March 25, 2014
A xenon-129 biosensor for monitoring MHC-peptide interactions
Andreas Schlundt1, Wolfgang Kilian, Michael Beyermann
1Protein Engineering Group, Leibniz-Institut für Molekulare Pharmakologie und FU Berlin, Robert-Roessle-Strasse 10, 13125 Berlin, Germany.
Angewandte Chemie (International Ed. in English)
|May 2, 2009
Abstract:
Caged in: The formation of a complex between a peptide ligand and a major histocompatibility complex (MHC) class II protein is detected by a (129)Xe biosensor. Cryptophane molecules that trap Xe atoms are modified with a hemagglutinin (HA) peptide, which binds to the MHC protein. The interaction can be monitored by an NMR chemical shift change of cage-HA bound (129)Xe.
