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Updated: Jun 23, 2026
An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
Published on: May 12, 2020
[Acute cytotoxicity of alternariol on NIH/3T3 cells]
Jimin Zhao1, Junfen Ma, Jun Zhao
1Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052,China. zhaojimin@zzu.edu.cn
Objective:
To study the cytotoxicity of altemariol (AOH) on NIH/3T3 cells.
Methods:
Logarithmic growth phase NIH/3T3 cells were treated at the dose of 10 micromol/L and 50 micromol/L AOH as treatment groups and treated with DMSO (0.25%). The effects of AOH on cell proliferations were assessed by morphologic observasion. Inhibition rates of AOH were determined by MTT assay. Comet assay was used to examine DNA damage induced by AOH. Cell cycle distributions were detected by flow cytometric assay (FCM).
Results:
The cells treated with AOH occured morlogic changes. The inhibition rates of 10 micromol/L and 50 micromol/L AOH were 19.88% and 32.47% respectively. In the comet assay two treatment groups percentages of tailed cells were 35.87% and 71.83% respectively. The DNA contents of the tail were (36.18 +/- 18.6) and (51.3 +/- 21.6) respectively. These datas were more higher than those of the solvent control (P < 0.05). In comparison with the control group, the percents of G2/M and S phase cells were increased after treatment of 50.0 micromol/L AOH for 24h (P < 0.05).
Conclusion:
AOH could have acute cytotoxic effects on NIH/3T3 cells and inhibite cell proliferation and cause DNA damage. High dose of AOH could induce cell cycle arrest in G2/M phase.
