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Updated: Jun 23, 2026
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Published on: May 4, 2021
[Effect of rat recombinant leptin on expression of SOCS-3 in mature adipocytes]
1School of Public Health, China Medical University, Shenyang 110001, China. liuli@mail.cmu.edu.cn
Objective:
To study the effect of rat recombinant leptin on expressions of suppressor of cytokine signaling 3 (SOCS-3) mRNA and protein in mature adipocytes .
Methods:
Mature adipocytes were separated from epididymal adipose tissues of male SD rats, and the expressions of long-form leptin receptor mRNA were detected. The separated adipocytes were then treated with rat recombinant leptin at the doses of 0.5-500 nmol/L for 0.5 to 6h. The expressions of SOCS-3 mRNA were measured with RT-PCR and proteins were measured with immuniprecipitation combined with Western blot analysis.
Results:
Low-level expressions of long-form leptin receptor mRNA could be measured in mature adipocytes. The adipocytes expressed small amounts of SOCS-3 mRNA and protein witbout leptin treatment, and no significant changes were observed at 0.5 Snmol/L of leptin. However, tbe expressions of SOCS-3 mRNA and protein increased significantly at the doses of 5-500 nmol/L of leptin in the consistent with treatment time.
Conclusion:
Leptin could have direct effects on adipocytes. High leptin concentration could induce the expressions of SOCS-3 mRNA and protein in mature adipocytes.
