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Updated: Jun 23, 2026
Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
Published on: March 12, 2021
[Study on PPARgamma and aP2 mRNA expression of adipose tissues in obesity and obesity resistant rats]
1School of Public Health, China Medical University, Shenyang 110001, China.
Objective:
To observe the difference of serum triglyceride, total cholesterol levels and expression of PPARgamma and aP2 mRNA of adipose tissues between obesity and obesity resistant rats induced by high-fat diet.
Methods:
Thirty-one healthy male Wistar rats were randomly divided into two groups. Seven of them were fed with standard diet as control group and 24 rats were fed with high-fat diet as high-fat group. At the end of the 8th week, according to the body weight increment, five rats were selected as obesity group, and five rats were selected as obesity resistant group from high-fat feeding rats. Serum triglyceride, total cholesterol, and the expressions of PPARgamma and aP2 mRNA of epididymal adipose tissues were detected.
Results:
Serum triglyceride of obesity group were higher than those of control (P < 0.05). Total cholesterol levels of obesity group were higher than those of control and obesity resistant group (P < 0.05, P < 0.01). The expressions of PPARgamma and aP2 mRNA of obesity group were also much higher than those of control and obesity resistant group.
Conclusion:
The expressions of PPARgamma and aP2 mRNA of obesity and obesity resistant rats induced by high-fat diet existed difference.
