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Related Experiment Video

Updated: Jun 23, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

In situ microarray fabrication and analysis using a microfluidic flow cell array integrated with surface plasmon

Jianping Liu1, Mark A Eddings, Adam R Miles

  • 1Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.

Analytical Chemistry
|May 5, 2009
PubMed
Summary
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This study integrates microfluidic flow cell arrays with Surface Plasmon Resonance Microscopy for high-throughput biomolecule analysis. The combined system enables rapid, label-free detection of interactions, advancing immunogenicity assays and drug discovery.

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Microfluidics

Background:

  • Surface Plasmon Resonance Microscopy (SPRM) offers label-free, real-time biomolecule interaction analysis.
  • Limitations in flow cell design and microarray fabrication have restricted SPRM's throughput and applications.

Purpose of the Study:

  • To integrate a microfluidic flow cell array (MFCA) with SPRM.
  • To enable in situ microarray fabrication and multichannel analysis of biomolecule interactions.
  • To develop a high-throughput, label-free immunogenicity assay.

Main Methods:

  • Integration of MFCA with SPRM for simultaneous microarray fabrication and analysis.
  • Utilizing 24 parallel channels for continuous sample delivery and real-time monitoring.
  • Label-free measurement of antibody-antibody interactions.

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Related Experiment Videos

Last Updated: Jun 23, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Main Results:

  • Demonstrated in situ probe antibody immobilization and real-time target antibody binding monitoring in 24 channels.
  • Achieved a limit of detection of 80 ng/mL for antibody-antibody interactions, significantly lower than industry standards.
  • Established the foundation for a high-throughput, label-free immunogenicity assay.

Conclusions:

  • The integrated MFCA-SPRM system enhances throughput and versatility for array-based analyses.
  • This technology is powerful for biomarker screening and drug discovery.
  • The system represents a significant advancement in label-free analytical tools for biomolecular studies.