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Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Published on: August 6, 2019

Lysine-based peptide-functionalized PLGA foams for controlled DNA delivery.

Hemin Nie¹, Shih Tak Khew, Lai Yeng Lee

¹Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore.

Journal of Controlled Release : Official Journal of the Controlled Release Society

|May 5, 2009

Summary

Modifying poly(lactic-co-glycolic acid) (PLGA) foams with lysine peptides enhances DNA delivery. This charge modification improves DNA adsorption and sustained release, proving beneficial for tissue engineering applications.

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Area of Science:

Biomaterials Science
Gene Delivery
Tissue Engineering

Background:

Poly(lactic-co-glycolic acid) (PLGA) scaffolds exhibit hydrophobicity and negative surface charges, hindering controlled release and tissue engineering.
Surface modification is crucial to overcome these limitations for enhanced biomaterial functionality.

Purpose of the Study:

To investigate the impact of surface charge modification on PLGA micro-porous foams for DNA delivery and transfection.
To evaluate the effects of tailored lysine peptides (K4 and K20) on DNA adsorption, release kinetics, and transfection efficiency.

Main Methods:

PLGA micro-porous foams were functionalized with K4 and K20 peptides using EDC/NHS crosslinkers.
X-ray photoelectron spectroscopy (XPS) confirmed peptide conjugation and DNA adsorption.
DNA adsorption capacity, release profiles, and DNA transfection (using BMP2 plasmid) were analyzed.

Main Results:

DNA adsorption and release were significantly influenced by the surface charge modification of PLGA foams.
K4- and K20-functionalized foams demonstrated more sustained DNA release compared to unmodified foams.
Sustained DNA release resulted in negligible cytotoxicity and prolonged gene expression, indicating improved DNA delivery.

Conclusions:

Surface charge modification of PLGA foams with lysine peptides is an effective strategy for enhancing DNA delivery.
The modified PLGA foams show promise as efficient and safe DNA delivery devices for tissue engineering applications.