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Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

Updated: Jun 23, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

Development of quadrupole mass spectrometers using rapid prototyping technology.

Boris Brkić1, Neil France, Adam T Clare

  • 1Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, United Kingdom. Boris.Brkic@liv.ac.uk

Journal of the American Society for Mass Spectrometry
|May 5, 2009
PubMed
Summary

Researchers developed a 3D-printed quadrupole mass filter (QMF) using digital light processing (DLP). This low-cost, miniature mass analyzer successfully analyzed light ions, demonstrating its potential for portable mass spectrometry.

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Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
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Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

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Last Updated: Jun 23, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
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Published on: August 28, 2021

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

Area of Science:

  • Analytical Chemistry
  • Instrument Development
  • Materials Science

Background:

  • Quadrupole mass filters (QMFs) are essential analytical instruments.
  • Traditional fabrication methods can be costly and complex.
  • Rapid prototyping offers potential for miniaturization and cost reduction.

Purpose of the Study:

  • To present a prototype design of a 3D-printed QMF using digital light processing (DLP).
  • To demonstrate the feasibility of DLP for fabricating hyperbolic electrodes for mass analyzers.
  • To validate the performance of the DLP QMF for low-mass ion analysis.

Main Methods:

  • Fabrication of a QMF prototype with hyperbolic electrodes using DLP 3D rapid prototyping.
  • Experimental analysis of H(2)(+), D(2)(+), and He(+) ions to obtain mass spectra.
  • Numerical simulations using CPO and Liverpool QMS-2 programs to model ion source and mass filter.
  • Performance evaluation of the DLP QMF for individual spectral peaks.

Main Results:

  • Successful proof-of-principle demonstration of the DLP QMF as a mass analyzer for low-mass ions (1-10 amu).
  • Experimental mass spectra obtained for H(2)(+), D(2)(+), and He(+) ions.
  • Instrument performance matched theoretical predictions from numerical simulations.
  • Demonstrated low-cost, lightweight, and miniaturized mass spectrometer potential.

Conclusions:

  • Digital light processing (DLP) is a viable technique for fabricating miniature quadrupole mass filters.
  • 3D rapid prototyping enables cost-effective production of mass analyzers with optimal geometries.
  • DLP facilitates the development of portable and microscale mass spectrometry applications.