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Updated: Jun 23, 2026

In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
ADP-ribosylation factor like 7 (ARL7) interacts with alpha-tubulin and modulates intracellular vesicular transport
Shu-mei Wei1, Chuan-gao Xie, Yasuhito Abe
1Department of Pathology, Second Affiliated Hospital of Zhejiang University, School of Medicine, Jiefang Road 88[#], Hangzhou, Zhejiang Province 310009, China.
Abstract:
ADP-ribosylation factor (ARF) like 7 (ARL7, also named ARL4C) is a member of ARL family and recent studies showed that it is involved in the AI-dependent cholesterol secretion process. Yet its biological function remains largely unknown. Using a MALDI-TOF/MS analysis, we identified alpha-tubulin interacted with ARL7. The interaction was confirmed by GST pull-down assay and co-immunoprecipitation in renal carcinoma cell 786-O in which we found the endogenous ARL7 is expressed. This is the second ARL member found interacting with tubulin after ARL8. In addition, ARL7Q72L, a GTP-binding form, promoted the transferrin transport from early endosome to recycling endosome significantly. The above data suggested that ARL7 might modulate the intracellular vesicular transport via interaction with microtubules.
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