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Updated: Jun 23, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
Exon junction complex enhances translation of spliced mRNAs at multiple steps
Hyung Chul Lee1, Junho Choe, Sung-Gil Chi
1School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Abstract:
Translation of spliced mRNAs is enhanced by exon junction complex (EJC), which is deposited on mRNAs as a result of splicing. Although this phenomenon itself is well known, the underlying molecular mechanism remains poorly understood. Here we show, using siRNAs against Y14 and eIF4AIII and spliced or intronless constructs that contain different types of internal ribosome entry sites (IRESes), that Y14 and eIF4AIII increase translation of spliced mRNAs before and after formation of the 80S ribosome complex, respectively. These results suggest that EJC modulates translation of spliced mRNA at multiple steps.
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