Bioactive phenols in algae: the application of pressurized-liquid and solid-phase extraction techniques.
L Onofrejová1, J Vasícková, B Klejdus
1Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic.
Summary
A novel pressurized-liquid with solid-phase extraction (PLE-SPE) method efficiently extracts phenolic compounds from algae. These extracts reveal valuable bioactive phenols in both freshwater and marine algae, offering insights into their potential health benefits.
Area of Science:
- Analytical Chemistry
- Phytochemistry
- Food Science
Background:
- Algae are recognized sources of bioactive compounds with potential health benefits.
- Efficient extraction methods are crucial for isolating and quantifying these valuable compounds.
- Phenolic compounds in algae contribute to their antioxidant properties.
Purpose of the Study:
- To develop and validate a new off-line pressurized-liquid with solid-phase extraction (PLE-SPE) technique.
- To extract and analyze phenolic acids and hydroxybenzaldehydes from freshwater and marine algae.
- To determine the total phenol content and antioxidant activity of algal extracts.
Main Methods:
- Off-line combination of pressurized-liquid extraction (PLE) and solid-phase extraction (SPE).
- High-performance liquid chromatography (HPLC) coupled with quadrupole mass spectrometry (MS) for identification and quantification.
- Folin-Ciocalteu assay for total phenol content and TEAC assay for antioxidant activity.
Main Results:
- The PLE-SPE method successfully extracted phenolic acids and hydroxybenzaldehydes from various algae.
- Submicrogram to microgram levels of target phenols were detected per gram of lyophilized algal sample.
- Algal extracts exhibited significant total phenol content and antioxidant capacity.
Conclusions:
- The developed PLE-SPE method is effective for analyzing bioactive phenols in algae.
- Freshwater and marine algae are valuable sources of diverse phenolic compounds.
- The findings support the potential use of these algae as sources of natural antioxidants.
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