Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
09:28

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Published on: April 19, 2010

Flow-based microfluidic device for quantifying bacterial chemotaxis in stable, competing gradients.

Derek L Englert1, Michael D Manson, Arul Jayaraman

  • 1Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, 77843-3122, USA.

Applied and Environmental Microbiology
|May 5, 2009
PubMed
Summary

Researchers studied bacterial chemotaxis using a new microfluidic device. They found that Escherichia coli

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated analyses of the microbiome, metabolome, and spatial transcriptomics reveal sex-dependent bladder vulnerability to chronic inorganic arsenic.

Journal of hazardous materials·2026
Same author

Aryl hydrocarbon receptor nuclear translocator 2: a forgotten per-ARNT-Sim transcription factor.

Toxicological sciences : an official journal of the Society of Toxicology·2026
Same author

Flagellar rotation comes full circle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Pesco-Vegetarian Food Components Promote Colonocyte Ferroptosis in Preclinical Mouse Models and a Randomized Crossover Trial in Healthy Human Adults.

The Journal of nutrition·2025
Same author

Interplay between dietary fiber, macrophages and colonocytes in a microfluidic model of host-microbiota interactions in colorectal cancer.

Lab on a chip·2025
Same author

A Chalcone Synthase-like Bacterial Protein Catalyzes Heterocyclic C-Ring Cleavage of Naringenin to Alter Bioactivity Against Nuclear Receptors in Colonic Epithelial Cells.

Metabolites·2025

Area of Science:

  • Microbiology
  • Biophysics
  • Chemical Engineering

Background:

  • Bacterial chemotaxis is crucial for understanding cell migration in chemical gradients.
  • Investigating responses to multiple competing signals is challenging with conventional methods.
  • Developing precise tools for gradient generation is essential for studying complex chemotactic behaviors.

Purpose of the Study:

  • To develop a microfluidic device for generating stable, precise chemical gradients.
  • To investigate the chemotactic responses of Escherichia coli to individual and combined chemoeffector gradients.
  • To elucidate the role of specific signaling molecules in bacterial migration.

Main Methods:

  • Utilized a microfluidic device employing laminar flow-based diffusive mixing to create chemical gradients.

More Related Videos

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Published on: October 13, 2019

Studies of Bacterial Chemotaxis Using Microfluidics - Interview
10:35

Studies of Bacterial Chemotaxis Using Microfluidics - Interview

Published on: May 28, 2007

Related Experiment Videos

Last Updated: Jun 23, 2026

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
09:28

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Published on: April 19, 2010

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Published on: October 13, 2019

Studies of Bacterial Chemotaxis Using Microfluidics - Interview
10:35

Studies of Bacterial Chemotaxis Using Microfluidics - Interview

Published on: May 28, 2007

  • Quantified bacterial migration using fluorescence microscopy to track Escherichia coli expressing green fluorescent protein.
  • Assessed responses to autoinducer-2 (AI-2), indole, and isatin, both individually and in combination.

    • Main Results:

    • Escherichia coli exhibited attraction to autoinducer-2 (AI-2) and repulsion from indole.
    • Cells were also attracted to isatin, an oxidized derivative of indole.
    • In competing gradients, attraction to AI-2 predominated over repulsion from indole.

    Conclusions:

    • The developed microfluidic device enables precise control over chemical gradients for chemotaxis studies.
    • Escherichia coli integrates attractive and repellent signals, with AI-2 overriding indole repulsion.
    • Concentration-dependent signal interactions are key factors in bacterial gut colonization.