Automated unrestricted multigene recombineering for multiprotein complex production
Related Experiment Video
Updated: Jun 23, 2026

The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
Christoph Bieniossek1, Yan Nie, Daniel Frey
1European Molecular Biology Laboratory, Grenoble Outstation, Grenoble, France.
Abstract:
Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon.
Related Concept Videos
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Recombinant DNA
Recombinant DNA
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Homologous Recombination
