Use of trypan blue stain in endothelial keratoplasty

We used the selective staining properties of trypan blue to demonstrate the efficacy and safety of the stain during endothelial keratoplasty (EK) surgery in 55 eyes of 51 patients. No long-term effects were seen due to the trypan blue staining up to two years postoperatively. Trypan blue facilitates the viewing and placement of the donor corneal disc into the recipient anterior chamber through a patient's cloudy cornea.