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Forward Genetic Approaches in Chlamydia trachomatis
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Forward Genetic Approaches in Chlamydia trachomatis

Published on: October 23, 2013

Real-time high resolution melting PCR for identification of the Swedish variant of Chlamydia trachomatis

Sophie Raherison1, Maithé Clerc, Sabine Trombert

  • 1Laboratoire de Bactériologie EA3671, Centre National de Référence des infections à Chlamydia, Université Victor Segalen Bordeaux 2, France.

Journal of Microbiological Methods
|May 6, 2009
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...