A simple method for monitoring protein-DNA interactions
Annabelle Fülöp1, Dumitru Arian, Andriy Lysenko
1Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
Bioorganic & Medicinal Chemistry Letters
|May 6, 2009
Abstract:
A simple, efficient and cheap method is reported for monitoring interactions between single stranded desoxyribonucleic acids and proteins, using fluorescence spectroscopy and complexes of 5'-dye-DNA conjugates with bovine serum albumin as probes. In the presence of a single stranded DNA-binding protein the complexes with bovine serum albumin are disrupted, which results in a reduction of fluorescence intensity.


