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Human Skeletal Muscle Biopsy Procedures Using the Modified Bergstr&#246;m Technique
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Published on: September 10, 2014

High-intensity exercise decreases muscle buffer capacity via a decrease in protein buffering in human skeletal

David Bishop1, Johann Edge, Alberto Mendez-Villanueva

  • 1School of Human Movement and Exercise Science, The University of Western Australia, Crawley, WA, 6009, Australia. David.Bishop@univr.it

Pflugers Archiv : European Journal of Physiology
|May 6, 2009
PubMed
Summary
This summary is machine-generated.

High-intensity exercise acutely reduces muscle buffer capacity (betam(in vitro)), primarily due to decreased protein buffering. This effect is consistent across exercise types and training interventions, impacting how muscle buffering is measured.

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Area of Science:

  • Exercise Physiology
  • Skeletal Muscle Metabolism
  • Biochemistry

Background:

  • Muscle buffer capacity (betam(in vitro)) is crucial for high-intensity exercise performance.
  • Previous research indicated an acute decrease in betam(in vitro) post-exercise.
  • The specific muscle buffers affected and the influence of exercise type and training remain unclear.

Purpose of the Study:

  • To identify which muscle buffers are affected by acute high-intensity exercise.
  • To determine the impact of exercise type (endurance vs. team sport) on these changes.
  • To investigate the effects of an interval training intervention on muscle buffering capacity.

Main Methods:

  • Muscle biopsies were obtained from male endurance and team-sport athletes.
  • Whole muscle and non-protein betam(in vitro) were measured at rest and immediately post-exercise.
  • Team-sport athletes underwent an interval training intervention, with measurements taken before and after.

Main Results:

  • High-intensity exercise significantly decreased betam(in vitro) in both athlete groups.
  • This decrease was primarily attributed to reduced protein buffering capacity.
  • Non-protein buffering capacity remained unchanged acutely post-exercise.
  • Training increased resting betam(in vitro) but did not alter the post-exercise decrease.

Conclusions:

  • Acute high-intensity exercise reduces muscle betam(in vitro) due to decreased protein buffering, irrespective of exercise type or training status.
  • Resting and post-exercise muscle betam(in vitro) are not interchangeable for measurement.
  • Researchers must account for acute exercise-induced decreases when assessing training adaptations in muscle buffering capacity.