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Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Related Experiment Video

Updated: Jun 23, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Sample preparation issues for tissue imaging by imaging MS.

Başak Kükrer Kaletaş¹, Ingrid M van der Wiel, Jonathan Stauber

¹PCC UvA B. V. , and FOM Institute for Atomic and Molecular Physics, Kruislaan 407, Amsterdam, The Netherlands.

|May 6, 2009

Summary

This summary is machine-generated.

Imaging mass spectrometry (MS) offers parallel chemical and spatial surface analysis without labels. This review highlights crucial sample preparation for reliable results in MALDI and secondary ion MS imaging experiments.

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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

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Last Updated: Jun 23, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

Area of Science:

Proteomics
Analytical Chemistry
Biotechnology

Background:

Imaging mass spectrometry (MS) is a rapidly advancing technique in proteomics.
It enables parallel, label-free spatial localization of multiple compounds on surfaces.
Complementary MS methods like MALDI and secondary ion MS imaging analyze the same tissue sample.

Purpose of the Study:

To review and discuss the importance of sample preparation in imaging MS.
To describe experimental requirements for optimal sample preparation in MALDI and secondary ion MS imaging.
To emphasize the impact of sample treatment on data quality and reproducibility.

Main Methods:

Discussion of sample collection and storage protocols.
Analysis of surface modification techniques for MS imaging.
Review of complementary imaging MS methods (MALDI and secondary ion MS).

Main Results:

Sample preparation is critical for high-quality, reproducible molecular images.
Optimizing each step, from collection to surface modification, is essential.
Proper sample treatment ensures reliable identification of small molecules, peptides, and proteins.

Conclusions:

Correct sample treatment is paramount for successful MALDI and secondary ion MS imaging.
Adherence to optimized experimental requirements enhances data reliability.
Advanced sample preparation techniques are key to unlocking the full potential of imaging MS in proteomics.