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Related Experiment Video

Updated: Jun 23, 2026

Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
06:17

Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues

Published on: August 25, 2020

Adipose stem cell side population in the mouse.

Thomas V Ramos1, Tracy Wang, Chad B Maki

  • 1PrimeGen Biotech LLC, Irvine, CA 92618, USA. tramos@primegenbiotech.com

Journal of Tissue Engineering and Regenerative Medicine
|May 6, 2009
PubMed
Summary

Researchers discovered side population (SP) stem cells in adult adipose tissue. These easily accessible stem cells can differentiate in vitro and regenerate skin in vivo, offering potential for surgical applications.

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Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Adipose tissue is a potential source of adult stem cells with significant plasticity.
  • Harvesting adult bone marrow stem cells presents challenges, making alternative sources desirable.
  • Side population (SP) cells, identified by Hoechst 33342 dye, are stem cells found in various tissues.

Purpose of the Study:

  • To identify and characterize side population (SP) stem cells in adult adipose tissue.
  • To evaluate the differentiation potential and in vivo regenerative capacity of adipose SP cells.

Main Methods:

  • Flow cytometry using Hoechst 33342 dye to identify and isolate SP cells from mouse adipose tissue.
  • In vitro cell culture to assess differentiation into osteogenic, chondrogenic, and adipogenic lineages.

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Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue
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Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

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Last Updated: Jun 23, 2026

Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
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Published on: August 25, 2020

Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue
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Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Published on: January 12, 2024

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

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  • In vivo studies using NOD/SCID mice to evaluate engraftment and wound healing capabilities.

    • Main Results:

    • The study identified approximately 2.5% adipose SP cells within the stromal vascular fraction of mouse adipose tissue.
    • Isolated mouse adipose SP cells demonstrated in vitro differentiation into multiple cell lineages.
    • Freshly sorted adipose SP cells engrafted in NOD/SCID mice and promoted skin regeneration with minimal scarring.

    Conclusions:

    • Adipose tissue harbors a population of stem cells (SP cells) with multipotent differentiation capacity.
    • Adipose SP cells show promise for in vivo tissue regeneration, particularly in dermal applications.
    • These findings highlight the potential of adipose-derived SP cells as a valuable resource for regenerative medicine and surgical therapies.