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New cryoprotectant for cryorefractive surgery.
K Kratz-Owens1, J W Huff, D J Schanzlin
1Bethesda Eye Institute, Department of Ophthalmology, St. Louis University School of Medicine, Missouri 63110.
Journal of Cataract and Refractive Surgery
|September 1, 1991
Summary
CPTES cryoprotectant significantly improves keratocyte survival after freeze injury compared to KM-26. This finding suggests CPTES may enhance recovery from cryorefractive surgeries by preserving corneal cells.
Area of Science:
- Ophthalmology
- Corneal Surgery
- Cryobiology
Background:
- Cryorefractive surgeries like keratomileusis can damage keratocytes, leading to corneal haze.
- Keratocyte destruction is a significant complication following these procedures.
Purpose of the Study:
- To evaluate the efficacy of two cryoprotectants, CPTES and KM-26, in preserving keratocyte survival after freeze injury.
- To compare the ability of CPTES and KM-26 to reduce postoperative corneal haze.
Main Methods:
- Corneal tissue was exposed to CPTES or KM-26 for varying durations before freezing.
- Keratocyte viability was assessed post-thaw.
- A rabbit model of lamellar keratoplasty was used to evaluate corneal haze post-surgery.
Main Results:
- CPTES immersion for five minutes resulted in 66.5% keratocyte survival, significantly higher than KM-26 (27.5%).
- CPTES maintained 40-80% of fresh tissue viability after freezing, while KM-26 maintained only 20-60%.
- Postoperative corneal haze in rabbits mirrored cell culture findings, favoring CPTES.
Conclusions:
- CPTES demonstrates superior cryoprotective properties for keratocytes compared to KM-26.
- CPTES may improve outcomes and speed recovery from cryorefractive surgeries by enhancing cell survival.