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New cryoprotectant for cryorefractive surgery.

K Kratz-Owens1, J W Huff, D J Schanzlin

  • 1Bethesda Eye Institute, Department of Ophthalmology, St. Louis University School of Medicine, Missouri 63110.

Journal of Cataract and Refractive Surgery
|September 1, 1991
PubMed
Summary
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CPTES cryoprotectant significantly improves keratocyte survival after freeze injury compared to KM-26. This finding suggests CPTES may enhance recovery from cryorefractive surgeries by preserving corneal cells.

Area of Science:

  • Ophthalmology
  • Corneal Surgery
  • Cryobiology

Background:

  • Cryorefractive surgeries like keratomileusis can damage keratocytes, leading to corneal haze.
  • Keratocyte destruction is a significant complication following these procedures.

Purpose of the Study:

  • To evaluate the efficacy of two cryoprotectants, CPTES and KM-26, in preserving keratocyte survival after freeze injury.
  • To compare the ability of CPTES and KM-26 to reduce postoperative corneal haze.

Main Methods:

  • Corneal tissue was exposed to CPTES or KM-26 for varying durations before freezing.
  • Keratocyte viability was assessed post-thaw.
  • A rabbit model of lamellar keratoplasty was used to evaluate corneal haze post-surgery.

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Main Results:

  • CPTES immersion for five minutes resulted in 66.5% keratocyte survival, significantly higher than KM-26 (27.5%).
  • CPTES maintained 40-80% of fresh tissue viability after freezing, while KM-26 maintained only 20-60%.
  • Postoperative corneal haze in rabbits mirrored cell culture findings, favoring CPTES.

Conclusions:

  • CPTES demonstrates superior cryoprotective properties for keratocytes compared to KM-26.
  • CPTES may improve outcomes and speed recovery from cryorefractive surgeries by enhancing cell survival.