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Related Experiment Video

Updated: Jun 23, 2026

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Published on: September 2, 2008

Nuclear envelope formation: mind the gaps.

Banafshé Larijani¹, Dominic L Poccia

¹Lincoln's Inn Fields Laboratories, Cancer Research UK, London WC2A 3PX, UK. banafshe.larijani@cancer.org.uk

Annual Review of Biophysics

|May 7, 2009

Summary

This summary is machine-generated.

Nuclear envelope (NE) assembly during cell division is complex. This review explores cytological, biophysical, and molecular models, emphasizing lipids and membrane fusion in NE formation.

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A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

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Published on: December 19, 2015

Area of Science:

Cell Biology
Molecular Biology
Biophysics

Background:

The nuclear envelope (NE) disassembles during prophase and reforms during telophase in metazoan mitosis.
Proper regulation of NE assembly is critical for accurate cell function.
Current understanding of NE formation mechanisms is incomplete, with uncertainty regarding a single unifying model.

Purpose of the Study:

To review cytological, biophysical, and molecular aspects of NE formation models.
To emphasize the role of lipids and lipid-modifying enzymes in NE assembly.
To explore membrane fusion mechanisms and the potential role of phosphoinositides in NE formation.

Main Methods:

Literature review integrating cytological, biophysical, and molecular data.
Analysis of membrane dynamics and fusion processes relevant to NE formation.
Speculative discussion on the role of specific lipids (phosphoinositides) in the process.

Main Results:

The NE serves as a model for studying membrane fusion dynamics.
Various fusion mechanisms contribute to the formation of the NE's double membrane.
Lipids and their modifying enzymes are key players in NE assembly.

Conclusions:

Multiple mechanisms likely contribute to NE formation during mitosis.
Lipid dynamics, particularly involving phosphoinositides, are crucial for membrane fusion and NE assembly.
Further research is needed to fully elucidate the complex process of NE formation.