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Updated: Jun 23, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

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Published on: March 8, 2020

Phospholipase D function in Saccharomyces cerevisiae.

Rima Mendonsa¹, JoAnne Engebrecht

¹Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA.

Biochimica Et Biophysica Acta

|May 7, 2009

Summary

This summary is machine-generated.

Phosphatidylcholine-specific phospholipase D is essential for yeast sporulation, mediating vesicle fusion via phosphatidic acid production. Its role in mitotic growth and mating is less understood, requiring further research.

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Defining Substrate Specificities for Lipase and Phospholipase Candidates

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Published on: November 23, 2016

Area of Science:

Cell Biology
Biochemistry
Molecular Biology

Background:

Phosphatidylcholine-specific phospholipase D (PC-PLD) is a conserved enzyme.
It plays a critical role in yeast sporulation, essential for prospore membrane formation.
PC-PLD regulates membrane fusion through phosphatidic acid production.

Purpose of the Study:

To elucidate the essential role of PC-PLD in yeast sporulation.
To investigate the non-essential roles of PC-PLD during mitotic growth and mating.
To understand the regulatory mechanisms of PC-PLD.

Main Methods:

Yeast genetics and molecular biology techniques.
Analysis of vesicle fusion and membrane dynamics.
Biochemical assays to study enzyme activity.

Main Results:

PC-PLD is indispensable for sporulation, facilitating Golgi and endosome vesicle fusion.
Phosphatidic acid, produced by PC-PLD, is crucial for membrane fusion and protein recruitment.
PC-PLD activity is dispensable during mitotic growth unless a major transfer protein is absent.
PC-PLD has a non-essential role in mating signal transduction.

Conclusions:

PC-PLD is vital for prospore membrane biogenesis in yeast.
The enzyme's regulation during sporulation and mitosis warrants further investigation.
PC-PLD's multifaceted roles highlight its importance in cellular membrane dynamics.