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Mating system and seed variation of Acacia hybrid (A. mangium x A. auriculiformis)
Chin-Hong Ng1, Soon-Leong Lee, Kevin Kit-Siong Ng
1Genetic Unit, Forest Biotechnology and Pharmaceutical Programme, Forest Research Institute Malaysia,52109 Kepong, Malaysia. chinhong@frim.gov.my
Abstract:
The mating system and seed variation of Acacia hybrid (A. mangium x A. auriculiformis) were studied using allozymes and random amplified polymorphic DNA (RAPD) markers, respectively. Multi-locus outcrossing rate estimations indicated that the hybrid was predominantly outcrossed (mean+/- s.e. t(m) = 0.86+/-0.01). Seed variation was investigated using 35 polymorphic RAPD fragments. An analysis of molecular variance (AMOVA) revealed the highest genetic variation among seeds within a pod (66%-70%), followed by among pods within inflorescence (29%-37%), and the least variation among inflorescences within tree (1%). In addition, two to four RAPD profiles could be detected among seeds within pod. Therefore, the results suggest that a maximum of four seeds per pod could be sampled for the establishment of a mapping population for further studies.
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