Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Preclinical Development: Overview

Preclinical Development: Overview

Preclinical development consists of a series of tests that ensure the safety and efficacy of a new therapeutic compound before it is tested in humans. There are four main phases to this process. First, safety pharmacology tests are conducted to ensure the drug does not produce any acutely harmful effects. These tests examine parameters such as bronchoconstriction, cardiac dysrhythmias, blood pressure changes, and ataxia. Next, preliminary toxicological testing is performed to determine the...

Clinical Trials: Overview

Clinical Trials: Overview

Clinical development focuses on how the drug will interact with the human body and encompasses four key phases of clinical trials, each serving a specific purpose in assessing the safety and effectiveness of new drugs. These phases overlap and build upon one another. Phase I involves a small group of healthy volunteers (typically 20-80 individuals) or, in cases where significant toxicity is expected, patients with the targeted disease, such as cancer or AIDS. The volunteers are tested for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rational engineering of the P5 TRS-mimic site and REP78/68 start codon yields promoter variants that improve rAAV purity while maintaining high titers.

Molecular therapy. Advances·2026

Same author

Cumulative dose of chemotherapy and survival outcomes in children with average-risk medulloblastoma treated on Children's Oncology Group study ACNS0331.

Neuro-oncology·2026

Same author

Developmental reprogramming underlies chemotherapy resistance in favorable-histology Wilms tumor.

Cell reports·2026

Same author

Capturing clinically actionable copy number alterations in Wilms tumor using nanopore sequencing.

Research square·2026

Same author

Target antigen-displaying extracellular vesicles boost CAR T cell efficacy in cell and mouse models of neuroblastoma.

Science translational medicine·2025

Same author

Paediatric Strategy Forum for medicinal product development of agents targeting GD2 ganglioside in children and adolescents with cancer.

European journal of cancer (Oxford, England : 1990)·2025

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

Published on: December 3, 2020

Initial testing of aplidin by the pediatric pre-clinical testing program.

Christopher L Morton¹, Peter J Houghton, Richard Gorlick

¹Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.

Pediatric Blood & Cancer

|May 7, 2009

Summary

Aplidin demonstrated potent in vitro activity against acute lymphoblastic leukemia (ALL) cell lines. In vivo studies showed Aplidin inhibited tumor growth in some models but did not cause regressions.

More Related Videos

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

Published on: April 3, 2026

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

Published on: December 7, 2021

Related Experiment Videos

Last Updated: Jun 23, 2026

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

Published on: December 3, 2020

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

Published on: April 3, 2026

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

Published on: December 7, 2021

Area of Science:

Pharmacology
Oncology
Cancer Research

Background:

Aplidin is a marine-derived compound with potential anticancer properties.
Previous research has explored its efficacy against various cancer types.

Purpose of the Study:

To evaluate the in vitro and in vivo anticancer activity of Aplidin.
To determine its efficacy against acute lymphoblastic leukemia (ALL) and solid tumor models.

Main Methods:

In vitro testing of Aplidin against cancer cell lines at concentrations from 0.1 nM to 1.0 microM.
In vivo testing in solid tumor and ALL xenograft models at a dose of 0.6 mg/kg.
Administration schedule involved intraperitoneal injection every 4 days x 3, repeated at day 21.

Main Results:

Aplidin exhibited highest in vitro activity against acute lymphoblastic leukemia (ALL) cell lines.
In vivo, Aplidin significantly impacted event-free survival (EFS) in 43% of solid tumor models (12/28).
Significant EFS differences were observed in 2 of 6 evaluable ALL models; tumor growth inhibition was noted, but no regressions occurred.

Conclusions:

Aplidin possesses potent in vitro anticancer activity, particularly against ALL.
The compound demonstrated significant in vivo tumor growth inhibition in certain xenograft models.
Further investigation is warranted to explore Aplidin's therapeutic potential and mechanisms of action.