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Decellularization and Recellularization of Whole Livers
Published on: February 4, 2011
Novel utilization of serum in tissue decellularization.
Liqiong Gui1, Stephen A Chan, Christopher K Breuer
1Department of Anesthesiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Tissue Engineering. Part C, Methods
|May 8, 2009
Summary
This study reveals that serum can effectively remove DNA from tissues during decellularization, preserving mechanical strength. This novel approach using serum offers broad applications in tissue engineering and regenerative medicine.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Decellularization is crucial for tissue engineering but faces limitations like altered matrix mechanics.
- Current methods are not universally applicable across all tissue types.
Purpose of the Study:
- To investigate the impact of serum on decellularization efficiency across diverse tissues.
- To identify potential improvements to existing decellularization protocols.
Main Methods:
- Detergent treatment followed by exposure to serum-containing media (endothelial growth media-2).
- Assessment of DNA and beta-actin removal.
- Mechanical strength evaluation using burst pressure.
- Testing on human umbilical artery, tissue-engineered vessels, and myocardium.
Main Results:
- Serum-containing media effectively removed DNA without eliminating cellular beta-actin.
- Tissue mechanical integrity, assessed by burst pressure, remained intact.
- The method proved effective on various tissue types, including engineered vessels and myocardium.
- Different serum types, including human serum, demonstrated DNA removal capabilities.
Conclusions:
- Serum represents a novel and effective agent for enhancing decellularization.
- This serum-based approach preserves tissue mechanics and is broadly applicable.
- The findings suggest a promising new direction for tissue decellularization techniques.
