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08:01
Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices
Published on: August 14, 2015
Cellular and molecular basis of tooth eruption
1Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. gwise@vetmed.lsu.edu
Orthodontics & Craniofacial Research
|May 8, 2009
Summary
The dental follicle regulates tooth eruption by controlling bone remodeling genes. It orchestrates bone resorption and formation chronologically and spatially for successful tooth movement.
Area of Science:
- Developmental Biology
- Craniofacial Development
- Molecular Biology
Background:
- Tooth eruption is a complex process necessitating coordinated bone remodeling.
- The dental follicle (DF) plays a crucial role in regulating alveolar bone resorption and formation.
- Understanding DF's molecular mechanisms is key to explaining eruption.
Purpose of the Study:
- To investigate how the dental follicle (DF) regulates osteoclastogenesis (bone resorption) and osteogenesis (bone formation) during tooth eruption.
- To determine the chronological and spatial gene expression patterns within the DF that control these processes.
Main Methods:
- Utilized multiple experimental methods to analyze gene expression and cellular activity.
- Examined gene expression in the rat 1st mandibular molar during postnatal development.
Main Results:
- DF regulates osteoclastogenesis and osteogenesis via differential gene expression, both chronologically and spatially.
- Major osteoclastogenesis (day 3) is mediated by CSF-1 and OPG, while minor osteoclastogenesis (day 10) involves VEGF and RANKL.
- Osteogenesis is promoted by BMP-2, with gene expression patterns supporting bone formation at the crypt base.
Conclusions:
- The dental follicle precisely controls the timing and location of bone remodeling required for tooth eruption.
- Differential gene expression in the DF, including CSF-1, OPG, VEGF, RANKL, and BMP-2, is critical for regulating osteoclastogenesis and osteogenesis.
- These findings elucidate the molecular basis of DF-mediated tooth eruption.
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