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Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Poly(2-N-carbazolylethyl acrylate)-modified silica as a new polymeric stationary phase for reversed-phase
Usha Ghimire Gautam1, Tsuyoshi Sawada, Mani Prasad Gautam
1Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
Abstract:
Poly(2-N-carbazolylethyl acrylate) having terminal trimethoxysilyl groups was newly synthesized by radical polymerization and immobilized onto the silica surface (Sil-CEA). The chromatographic property of Sil-CEA was examined by applying polycyclic aromatic hydrocarbons as solutes. Poly(4-vinylpyridine)-modified silica (Sil-VP) and monomeric octadecylated silica (ODS) columns were used as the reference columns. Less sensitivity to molecular hydrophobicity and enhanced molecular planarity selectivity were obtained with Sil-CEA compared to ODS. On the other hand, high retention factors for the analyzed solutes and an opposite elution order for linear and disc-shaped solutes were obtained with Sil-CEA compared with Sil-VP. In this paper, the application for separation of tocopherols was also described.
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