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A Toxicological and Ecotoxicological Assay Based on Mussel (Mytilus galloprovincialis) Hemocytes Motility
Published on: December 13, 2024
Studies on the interaction between benzidine and hemocyanin from Chinese mitten crab Eriocheir japonica sinensis
Bo-Ping Tang1, Yan-Qing Wang, Dai-Zhen Zhang
1Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Kaifang Dadao Road 50, Yancheng City, Jiangsu Province 224002, People's Republic of China. tangwang2008@163.com
Abstract:
The interaction of benzidine (BZ) with hemocyanin (Hc) from Chinese mitten crab (Eriocheir japonica sinensis) was studied by fluorescence spectrum. BZ can quench the fluorescence of Hc by forming a new complex. The process of binding BZ on Hc was a spontaneous molecular interaction procedure. The thermodynamic parameters, the enthalpy change and entropy change were estimated to be 47.36 kJ mol(-1), 248.51 J mol(-1) K(-1) according to the van' Hoff equation. This indicates that hydrophobic interaction played a major role in stabilizing the complex. The results of synchronous fluorescence spectroscopy and three-dimensional fluorescence spectra indicated that the structure of these tyrosine residues environments was altered by BZ, which could enter into the hydrophobic pocked of Hc.

