Generation of vero cells expressing ebola virus glycoprotein

To establish replication-incompetent Ebola virus (EBOV) lacking its glycoprotein (GP), we attempted to generate a Vero cell line that constitutively expressed GP. We used a retroviral vector to transduce Vero cells with the EBOV GP gene, resulting in a high expression level of GP on the cell surface. The Vero cells expressing EBOV GP complemented the replication cycle of vesicular stomatitis virus, which lacks the essential viral glycoprotein. This cell line might be useful for basic research on EBOV GP as well as for vaccine production.