Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Interaction of EM Radiation with Matter: Spectroscopy01:12

Interaction of EM Radiation with Matter: Spectroscopy

Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in its path. The electric field in the radiation can interact with electrical charges in the atoms or molecules in the matter. On the other hand, the magnetic field can interact with the magnetic field in the atomic nucleus. The study of the interaction between electromagnetic radiation and matter is termed spectroscopy. Spectroscopy is the study...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-time processing of high-throughput quantitative phase microscopy data using a Jetson Orin Nano.

Biophotonics discovery·2026
Same author

Enhanced penetration depth in optical coherence tomography and photoacoustic microscopy <i>in vivo</i> enabled by absorbing dye molecules.

Optica·2026
Same author

Prospective Identification of Dysplasia in Barrett's Esophagus With Combined Optical Coherence Tomography and Light Scattering Measurements.

Journal of biophotonics·2025
Same author

Spectroscopic analysis of volumetric OCT data for the automated measurement of scatterer size.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025
Same author

Indocyanine green (ICG) enhances penetration of 1300 nm optical coherence tomography imaging for <i>in vivo</i> murine skin.

Optics letters·2025
Same author

High-Performance, Low-Cost Optical Coherence Tomography System Using a Jetson Orin Nano for Real-Time Control and Image Processing.

Translational vision science & technology·2025
Same journal

Transform-The Path Forward: Endoscopy and the Science of Expertise.

Gastrointestinal endoscopy clinics of North America·2026
Same journal

Ushering in a New Era of Training in Endoscopy.

Gastrointestinal endoscopy clinics of North America·2026
Same journal

Where Do We Go from Here: Transforming Teaching Endoscopy Knowledge Using the Expert Performance Approach.

Gastrointestinal endoscopy clinics of North America·2026
Same journal

Integrating Artificial Intelligence into Endoscopy Training.

Gastrointestinal endoscopy clinics of North America·2026
Same journal

From Expert to Educator: Translating Expert Performance into Teachable Competencies Through Trainer Development in Endoscopic Simulation.

Gastrointestinal endoscopy clinics of North America·2026
Same journal

Implementing Endoscopy Simulation Nationally.

Gastrointestinal endoscopy clinics of North America·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
06:50

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

Published on: December 2, 2017

Reflectance spectroscopy.

Michael B Wallace1, Adam Wax, David N Roberts

  • 1Mayo Clinic, Jacksonville, FL, USA. wallace.michael@mayo.edu

Gastrointestinal Endoscopy Clinics of North America
|May 9, 2009
PubMed
Summary
This summary is machine-generated.

Reflectance spectroscopy offers rapid, safe tissue evaluation for dysplasia and ischemia. This technology is used with endoscopes to detect conditions like Barrett's esophagus and colon polyps.

More Related Videos

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
07:05

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

Published on: June 18, 2021

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
10:20

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses

Published on: August 9, 2019

Related Experiment Videos

Last Updated: Jun 23, 2026

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
06:50

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

Published on: December 2, 2017

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
07:05

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

Published on: June 18, 2021

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
10:20

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses

Published on: August 9, 2019

Area of Science:

  • Biomedical optics
  • Medical diagnostics
  • Gastroenterology

Background:

  • Reflectance spectroscopy is an emerging technology for tissue analysis.
  • Current methods for detecting dysplasia and ischemia can be invasive or time-consuming.

Purpose of the Study:

  • To highlight the utility of reflectance spectroscopy in clinical settings.
  • To present reflectance spectroscopy as a rapid and safe diagnostic tool.

Main Methods:

  • Utilizes probe-based devices compatible with standard endoscopes.
  • Employs optical principles to analyze tissue characteristics.

Main Results:

  • Demonstrates rapid and safe evaluation of tissue for dysplasia and ischemia.
  • Successfully applied in detecting dysplasia in Barrett's esophagus, colitis, and colon polyps.

Conclusions:

  • Reflectance spectroscopy is a valuable tool for in-vivo tissue assessment.
  • Its integration with endoscopy enhances diagnostic capabilities for gastrointestinal conditions.