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Updated: Jun 23, 2026

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
[Low prevalence of Mycoplasma pneumoniae infection in children with acute pharyngitis]
María Fernández de Sevilla1, Josep Alayeto, Yolanda Fernández
1Agrupació Sanitària Hospital Sant Joan de Déu-Clínic, Universitat de Barcelona, Esplugues de Llobregat, España. mariafernandez@hsjdbcn.org
Insights
Mycoplasma pneumoniae plays a minimal role in pediatric acute pharyngitis, with low incidence observed. All healthy controls tested negative for M. pneumoniae, suggesting further evaluation of positive cases is needed.
Area of Science:
- Microbiology
- Pediatric Infectious Diseases
Context:
- Acute pharyngitis is a common pediatric illness.
- Mycoplasma pneumoniae is a potential pathogen.
- Understanding the causative agents of pharyngitis is crucial for appropriate treatment.
Purpose:
- To investigate the prevalence of Mycoplasma pneumoniae in children with acute pharyngitis.
- To compare M. pneumoniae detection rates between symptomatic children and healthy controls.
Summary:
- A prospective study enrolled 182 children with acute pharyngitis and 60 healthy controls.
- Polymerase Chain Reaction (PCR) was used to detect M. pneumoniae in pharyngeal samples.
- M. pneumoniae was detected in 3.8% of pediatric cases and in none of the controls.
Impact:
- The study indicates a low incidence of Mycoplasma pneumoniae-associated acute pharyngitis in the studied population.
- Negative results in healthy controls suggest M. pneumoniae may have limited pathogenicity in pharyngitis.
- Further investigation is warranted for PCR-positive M. pneumoniae cases in acute pharyngitis to determine clinical significance.
Introduction:
The aim of this study is to determine the role of Mycoplasma pneumoniae in pediatric patients with acute pharyngitis and in a healthy control group.
Methods:
Prospective study conducted April and June 2006 of children with acute pharyngitis. M. pneumoniae infection was determined by PCR in pharyngeal exudate specimens from these patients and a healthy control group.
Results:
A total of 182 cases and 60 controls were enrolled. PCR for M. pneumoniae was positive in 7 cases (3.8%, 95% CI 1.5-7.7) and negative in all controls.
Conclusions:
The incidence of acute pharyngitis due to M. pneumoniae is low in our setting. In healthy patients (control group) all M. pneumoniae testing by PCR was negative; therefore, the possible pathological significance of all cases of acute pharyngitis PCR-positive for M. pneumoniae should be evaluated.
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