Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Interference and Diffraction02:18

Interference and Diffraction

Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IMAGING REDOX STATE HETEROGENEITY WITHIN INDIVIDUAL EMBRYONIC STEM CELL COLONIES.

Journal of innovative optical health sciences·2021
Same author

Pushing the boundaries of optoacoustic microscopy by total impulse response characterization.

Nature communications·2020
Same author

Full-frequency correction of spatial impulse response in back-projection scheme using space-variant filtering for optoacoustic mesoscopy.

Photoacoustics·2020
Same author

Indocyanine green matching phantom for fluorescence-guided surgery imaging system characterization and performance assessment.

Journal of biomedical optics·2020
Same author

Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy.

Light, science & applications·2020
Same author

A Synthetic Total Impulse Response Characterization Method for Correction of Hand-Held Optoacoustic Images.

IEEE transactions on medical imaging·2020
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Analytical model for dual-interfering sources diffuse optical tomography.

Xavier Intes, Vasilis Ntziachristos, Britton Chance

    Optics Express
    |May 9, 2009
    PubMed
    Summary
    This summary is machine-generated.

    This study presents an analytical model for imaging absorptive inclusions in scattering media using dual interfering sources. The method accurately reconstructs objects, even multiple ones, at various depths.

    More Related Videos

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    Related Experiment Videos

    Last Updated: Jun 23, 2026

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
    12:24

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

    Published on: July 17, 2012

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    Area of Science:

    • Biomedical Optics
    • Medical Imaging
    • Photonic Imaging

    Background:

    • Highly scattering media pose challenges for traditional imaging techniques.
    • Accurate reconstruction of absorptive inclusions is crucial for medical diagnostics, particularly in breast imaging.
    • Contrast agents enhance visibility but require sophisticated reconstruction algorithms.

    Purpose of the Study:

    • To develop an analytical model for tomographic reconstruction of absorptive inclusions in highly scattering media.
    • To assess the performance and parameters affecting reconstruction quality using simulated breast-like phantom data.
    • To demonstrate the algorithm's capability in resolving multiple objects.

    Main Methods:

    • Derivation of an analytical model utilizing dual interfering sources.
    • Application of a first-order Rytov expansion perturbation approach to solve the diffusion equation.
    • Obtaining analytical weight functions for inverse problem solving.
    • Validation using simulated data of contrast-enhanced breast-like phantoms.

    Main Results:

    • Successful tomographic reconstructions of absorptive inclusions were achieved.
    • Reconstruction quality was evaluated based on object depth, modulation frequency, and source separation.
    • The algorithm demonstrated the ability to resolve multiple embedded objects.
    • The model proved effective for simulated contrast-enhanced breast tissue.

    Conclusions:

    • The developed analytical model provides an effective method for imaging absorptive inclusions in highly scattering media.
    • The Rytov expansion-based approach offers a robust solution for inverse problems in diffuse optical tomography.
    • The study highlights the potential for improved diagnostic capabilities in areas like breast cancer detection.