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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Tissue-specific expression of receptor-interacting protein in aging mouse
1Biochemistry and Molecular Biology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, 221005, India.
Age (Dordrecht, Netherlands)
|May 9, 2009
Summary
Receptor-interacting protein (RIP) expression varies by tissue, sex, and age in mice. RIP isoforms differ in abundance, decreasing with age in some tissues but increasing in others, suggesting diverse regulatory roles.
Area of Science:
- Molecular biology
- Endocrinology
- Biochemistry
Background:
- Receptor-interacting protein (RIP) is a known coregulator for nuclear receptors.
- Understanding RIP's expression patterns is crucial for elucidating its regulatory functions.
Purpose of the Study:
- To investigate the expression patterns of RIP isoforms in various mouse tissues.
- To determine how RIP expression is affected by age and sex.
Main Methods:
- Analysis of RIP protein expression using molecular weight characterization.
- Comparison of RIP levels across different tissues (liver, kidney, lung, adipose, prostate, testis).
- Assessment of age- and sex-dependent variations in RIP expression.
Main Results:
- Two RIP isoforms (140 kDa and 137 kDa) were detected in liver and kidney, while only the 140 kDa isoform was found in lung, adipose, prostate, and testis.
- RIP isoform levels decreased in the liver and kidney of older mice compared to adults.
- Kidney RIP140 expression was lower in older males than females, whereas adipose tissue showed significantly higher RIP140 levels in older mice of both sexes.
Conclusions:
- RIP expression is tissue-specific, sex-dependent, and age-influenced in mice.
- These variations suggest differential functional roles for RIP as a nuclear receptor coregulator across different physiological contexts.
