Related Experiment Video
Updated: Jun 23, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
Published on: February 4, 2011
Insulator-based dielectrophoretic separation of small particles in a sawtooth channel
Kang Ping Chen1, Jose Rafael Pacheco, Mark A Hayes
1Department of Mechanical and Aerospace Engineering, Arizona State University, Tempe, AZ 85287-6106, USA. k.p.chen@asu.edu
Abstract:
Insulator-based dielectrophoretic separation of small particles in a sawtooth channel is studied in the limit of dilute concentration. Pathlines for the movements of infinitesimal particles are constructed and the geometric changes of these pathlines are used to establish the criterion for blocking and trapping particles with different physical properties. The sharp corners of the sawtooth channel create much stronger dielectrophoretic force than channels with smooth corners for blocking particle movements. Particle blocking and trapping depend on particle properties and the geometry of the device. It is shown that once the channel geometric aspect ratios are specified, the blocking criterion depends on only a single dimensionless parameter C defined in terms of the particle mobility ratio (dielectrophoretic versus electrokinetic), the applied voltage and the spacing between the teeth. Selective blocking and trapping of particles can be realized by varying the geometry of the channel progressively. High-resolution separation can be achieved by tuning the differential in the parameter C to a desired level.
Related Concept Videos
Capillary Electrophoresis: Instrumentation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography
Electrophoresis: Overview
There...
Dielectric Polarization in a Capacitor
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

